Biochemical methods to assess CFTR expression and membrane localization

Intracellular CFTR: localization and function.

Intracellular CFTR: Localization and Function. Physiol. Rev. 79, Suppl.: S175-S191, 1999. - There is considerable evidence that CFTR can function as a chloride-selective anion channel. Moreover, this function has been localized to the apical membrane of chloride secretory epithelial cells. However, because cystic fibrosis transmembrane conductance regulator (CFTR) is an integral membrane protei...

specialized methods to teach spelling: comparing three methods

چکیده: بررسی ادبیات مربوطه در کشور در زمینه یادگیری زبان انگلیسی نشان می‎دهد که علی‎رغم اهمیت املا در فرآیند یادگیری به طور عام و یادگیری زبان انگلیسی به طور خاص، این مولفه از جایگاهی متناسب با اهمیت آن برخوردار نیست و عمدتاً نادیده گرفته شده است. تحقیقات گسترده‎ای در خارج از کشور در مورد ماهیت این مولفه صورت گرفته است، در حالی که به جرأت می‎توان گفت در داخل کشور گامی در مورد درک ماهیت آن و فرآی...

Transport of CFTR to the Plasma Membrane

The mechanism whereby cAMP stimulates Cl flux through CFTR ion channels in secretory epithelia remains controversial. It is generally accepted that phosphorylation by cAMP-dependent protein kinase increases the open probability of the CFTR channel. A more controversial hypothesis is that cAMP triggers the translocation of CFTR from an intracellular pool to the cell surface. We have monitored me...

RANTES Expression via Its C-Terminal Plasma Membrane CFTR Regulates

Electrophysiological, biochemical, and bioinformatic methods for studying CFTR channel gating and its regulation.

CFTR is the only member of the ABC (ATP-binding cassette) protein superfamily known to function as an ion channel. Most other ABC proteins are ATP-driven transporters, in which a cycle of ATP binding and hydrolysis, at intracellular nucleotide binding domains (NBDs), powers uphill substrate translocation across the membrane. In CFTR, this same ATP-driven cycle opens and closes a transmembrane p...